Journal: JCI Insight
Article Title: Dynamic transition of Tregs to cytotoxic phenotype amid systemic inflammation in Graves’ ophthalmopathy
doi: 10.1172/jci.insight.181488
Figure Lengend Snippet: ( A ) Bar chart showed the GO functional enrichment of downregulated DEGs in the corticosteroid-treated group compared with the GO group. ( B ) The cell proportions of CD4 T cell subpopulations within the GO and Treated groups. Data are represented as the median IQR. ** P < 0.001 by Mann-Whitney U test. ( C ) The expression and activity scores of Graves’ disease, inflammatory, and IFNG signal gene sets between the GO and Treated groups. Data are represented as the median IQR. **** P < 0.00001 by Mann-Whitney U test. ( D ) Flow density plots and dot plots showed the expression intensity of IL-6, TNF-α, and IFN-γ in peripheral blood PBMCs from the GO and Treated groups (GO, n = 9; Treated, n = 9). Data are represented as the median IQR. *** P < 0.0001 by Mann-Whitney U test. ** P adj < 0.001. ( E ) Violin plots show the CTL signature score of aTreg between the GO and Treated groups. Data are represented as the median IQR. **** P < 0.00001 by Mann-Whitney U test. ( F ) Chromatin accessibility plots for KLRC1 and KLRC4 genes in aTreg between the GO and Treated groups. ( G ) Flow cytometry plots displayed the expression of KLRC1 and FGFBP2 in peripheral blood CD4 + CD25 + FOXP3 + Treg in Treated groups; dot plots indicating the target cell proportions (GO, n = 9; Treated, n = 9). ** P adj < 0.001. Data are represented as the median IQR. *** P < 0.00001 by Mann-Whitney U test.
Article Snippet: Subsequently, corresponding flow cytometry antibodies were incubated in flow cytometry staining buffer (Proteintech, PF00018) to detect specific cell protein in human or mouse cells.
Techniques: Functional Assay, MANN-WHITNEY, Expressing, Activity Assay, Flow Cytometry